Monitoring ofSaccharomycesandHanseniasporapopulations during alcoholic fermentation by real-time quantitative PCR
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چکیده
منابع مشابه
Monitoring of Leuconostoc population during sauerkraut fermentation by quantitative real-time polymerase chain reaction.
A real-time PCR assay method was established to monitor Leuconostoc spp. populations via specific amplification of the dextransucrase gene. Quantification of L. mesenteroides B-512F using both genomic DNA and cell suspensions yielded a log-linear correlation spanning approximately 5 log units. By using this method, monitoring changes of Leuconostoc spp. during sauerkraut fermentation was succes...
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Those who have worked in the field of quantitative polymerase chain reaction (PCR) since the early 1990s have accepted many of the tedious aspects of the early assays as routine. Difficulties associated with early quantitative PCR techniques included: (i) ensuring that the PCR was within the linear range of amplification (that portion where the PCR signal is directly proportional to the input c...
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ژورنال
عنوان ژورنال: FEMS Yeast Research
سال: 2007
ISSN: 1567-1356,1567-1364
DOI: 10.1111/j.1567-1364.2007.00304.x